A RAB family antigen of Angiostrongylus cantonensis induces a Th1-type immune response in vitro and in vivo

作者：Chuang Chih Cheng; Chen Chi Wu; Su Kua Eyre; Tsai Yueh Ting; Du Wen Yuan^*

来源：Experimental Parasitology, 2013, 134(1): 122-127.

DOI：10.1016/j.exppara.2013.02.009

• Angiostrongylus cantonensis
• RAB-2
• Eosinophilic meningitis

A cDNA library was constructed from an Angiostrongylus cantonensis young adult and the encoded proteins were expressed in Escherichia coli. One reactive antigen, a RAB-2 protein, was selected using an immunoscreening technique. The expression of the Th1-type cytokine IFN-gamma was elicited in mouse splenic cells that were co-cultured with the recombinant RAB-2 protein and in the sera of mice that were immunised with this protein and adjuvant (50 mu g at 2-week intervals). In the A. cantonensis-infected groups, the mice were orally infected with 35 infective larvae, and a subset of the infected mice were immunised with the recombinant RAB-2 protein in adjuvant. Serum samples were collected every week for ELISA, and the pathological examinations were performed at 14 days post infection (dpi). An increase in IFN-gamma expression was noted in the blood, and the brain sections revealed moderate eosinophilic meningitis in the immunised mice. The RAB-2 antigen of A. cantonensis induced a Th1-type immune response both in vitro and in vivo.

• 出版日期2013-5