Analysis of cholesterol oxidation products by Fast gas chromatography/mass spectrometry

作者：Cardenia Vladimiro; Rodriguez Estrada Maria T^*; Baldacci Elena; Savioli Stefano; Lercker Giovanni

来源：Journal of Separation Science, 2012, 35(3): 424-430.

DOI：10.1002/jssc.201100660

• Cholesterol oxidation products
• Fast GC
• MS
• Mouse liver
• Oxysterols
• Sardine

The aim of the present study was to set-up a Fast gas chromatography/mass spectrometry method for the analysis of cholesterol oxidation products (COPs). A silylated mixture of seven oxysterol standards was injected into a Fast GC/MS system. A capillary GC column (10 m x0.1 mm internal diameter x 0.1 mu m film thickness) coated with 95% dimethyl- and 5% diphenyl-polysiloxane, was used. The method gave a fast (total analysis time=3.5 min) and satisfactory resolution (R%26gt;1.2) of the COPs standards, with a good repeatability and sensitivity, similar to those of conventional GC/MS; recoveries were tested on mice liver. Fast GC/MS method suitability for COPs analysis in food was also tested on an oxidized sardine fillet, which had been previously saponified and purified by NH2 solid-phase extraction (SPE); a good repeatability and sensitivity was also obtained. The analytical performance of the Fast GC/MS method for the determination of COPs, together with the consequent significant reduction of the analysis time and consumables, demonstrates that Fast GC/MS represents a valid alternative to conventional GC/MS and evinces the great potential of such an analytical technique, which could be applied for both food and biological samples.

• 出版日期2012-2