All-trans Retinoic Acid Promotes Cell Apoptosis Through Gamma Amino Butyric Acid Pathway in Murine Embryonic Palate Mesenchymal Cells

作者：Wei Xue Tao; Qiao Yang Zheng; Shang Lan Qin; Li Yong^*

来源：Progress in Biochemistry and Biophysics, 2012, 39(3): 264-272.

DOI：10.3724/SP.J.1206.2011.00199

• all-trans retinoic acid
• murine embryonic palate mesenchymal cell
• glutamic acid decarboxylase
• gamma amino butyric acid
• proliferation
• apoptosis

Retinoic acid (RA) is a teratogen which can induce cleft palate. Recent studies suggested that gamma amino butyric acid (GABA) was involved in the development of palate. If the GABA signal pathway participates the cleft palate induced by RA remains to be elucidated. In the present study, we investigated the effect of all-trans retinoic acid (atRA) (0.2, 0.67, 2.0 and 6.7 mu mol/L) on cell proliferation and apoptosis, and then examined the role of gamma amino butyric acid (GABA) signaling pathway in regulation of cell proliferation and apoptosis by atRA in murine embryonic palate mesenchymal (MEPM) cells. Results showed that atRA (2 mu mol/L and 6.7 mu mol/L) significantly inhibited cell proliferation and increased apoptosis. The mRNA and protein expression of glutamic acid decarboxylase 67 (GAD 67) which was a key enzyme in synthesis of GABA were significantly down regulated by atRA (0.67, 2.0 mu mol/L and 6.7 mu mol/L). But the mRNA and protein expression of GABAAR-133 were shown no obvious change compared with the control group. When GABA (1.0 mu mol/L) was added to cell culture system, the effect of atRA (6.7 mu mol/L) on the proliferation and apoptosis of MEPM cells was reversed. In conclusion, all-trans retinoic acid inhibits cell proliferation and promotes cell apoptosis through gamma amino butyric acid pathway in murine embryonic palate mesenchymal cells.

• 出版日期2012-3
• 单位北京大学