A defined long-term in vitro tissue engineered model of neuromuscular junctions

作者：Das Mainak; Rumsey John W; Bhargava Neelima; Stancescu Maria; Hickman James J^*

来源：Biomaterials, 2010, 31(18): 4880-4888.

DOI：10.1016/j.biomaterials.2010.02.055

• In vitro test
• Muscle
• Nerve tissue engineering
• Neural cell
• Surface modification
• Self assembly

Neuromuscular junction (NMJ) formation, occurring between motoneurons and skeletal muscle, is a complex multistep process involving a variety of signaling molecules and pathways. In vitro motoneuron-muscle co-cultures are powerful tools to study the role of different growth factors, hormones and cellular structures involved in NMJ formation. In this study, a serum-free culture system utilizing defined temporal growth factor application and a non-biological substrate resulted in the formation of robust NMJs. The system resulted in long-term survival of the co-culture and selective expression of neonatal myosin heavy chain, a marker of myotube maturation. NMJ formation was verified by colocalization of dense clusters of acetylcholine receptors visualized using alpha-bungarotoxin and synaptophysin containing vesicles present in motoneuron axonal terminals. This model will find applications in basic NMJ research and tissue engineering applications such as bio-hybrid device development for limb prosthesis and regenerative medicine as well as for high-throughput drug and toxin screening applications.

• 出版日期2010-6