A Novel Scoring System Combining Expression of CD23, CD20, and CD38 with Platelet Count Predicts for the Presence of the t(11;14) Translocation of Mantle Cell Lymphoma

作者：Medd Patrick G; Clark Nithiya; Leyden Kevin; Turner Susan; Strefford Jennifer A; Butler Caroline; Coffins Graham P; Roberts David J; Atoyebi Wale; Hatton Chris S R^*

来源：Cytometry Part B: Clinical Cytometry , 2011, 80B(4): 230-237.

DOI：10.1002/cyto.b.20590

• mantle cell lymphoma
• CD38
• CD23
• CD20
• thrombocytopenia
• FISH
• screening

Background: Both chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL) are CD5/19 positive. The t(11;14) MCL translocation is identified by fluorescent in situ hybridization (FISH) and can distinguish the two disorders. We attempted to identify flow cytometric and other markers predictive of a positive FISH test.
Methods: We examined 100 atypical CLL/MCL cases for demographic, hematological, and cytometric variables, 96 were FISH tested for t(11;14) and four were known MCL.
Results: Twenty-two cases were confirmed as MCL. Multivariate analysis identified four variables associated with MCL: Thrombocytopenia (taken as Plt < 150 x 10(9)/L), CD23 negative, CD20 strong, and CD38 positive, with these variables a four-point score was devised. By ROC analysis, the MCL score was superior in differentiating MCL to the Marsden CLL score (AUC 0.95 vs. 0.78). MCL score >= 2 showed sensitivity 1, specificity 0.66, positive predictive value (PPV) 0.49, and negative predictive value (NPV) 1 for MCL. The score was then prospectively validated on an independent cohort of 44 cases of atypical CLL/MCL. No MCL had a score <3. Validation PPV/NPV of score >= 3 were 0.5/1. Overall survival in MCL was shorter compared to t(11;14) negative patients (median 3.3 vs. 4.2 years, HR 2.2, 95% CI 0.87-5.5, P = 0.1).
Conclusions: The score described can be used to identify cases of CD5/19 positive lymphoproliferative disorders likely to be t(11;14) positive MCL.

• 出版日期2011-7