Activation of beta-Catenin Signaling in Androgen Receptor-Negative Prostate Cancer Cells

作者：Wan Xinhai; Liu Jie; Lu Jing Fang; Tzelepi Vassiliki; Yang Jun; Starbuck Michael W; Diao Lixia; Wang Jing; Efstathiou Eleni; Vazquez Elba S; Troncoso Patricia; Maity Sankar N; Navone Nora M^*

来源：Clinical Cancer Research, 2012, 18(3): 726-736.

DOI：10.1158/1078-0432.CCR-11-2521

Purpose: To study Wnt/beta-catenin in castrate-resistant prostate cancer (CRPC) and understand its function independently of the beta-catenin-androgen receptor (AR) interaction. %26lt;br%26gt;Experimental Design: We carried out beta-catenin immunocytochemical analysis, evaluated TOP-flash reporter activity (a reporter of beta-catenin-mediated transcription), and sequenced the beta-catenin gene in MDA prostate cancer 118a, MDA prostate cancer 118b, MDA prostate cancer 2b, and PC-3 prostate cancer cells. We knocked down beta-catenin in AR-negative MDA prostate cancer 118b cells and carried out comparative gene-array analysis. We also immunohistochemically analyzed beta-catenin and AR in 27 bone metastases of human CRPCs. %26lt;br%26gt;Results: beta-Catenin nuclear accumulation and TOP-flash reporter activity were high in MDA prostate cancer 118b but not in MDA prostate cancer 2b or PC-3 cells. MDA prostate cancer 118a and MDA prostate cancer 118b cells carry a mutated beta-catenin at codon 32 (D32G). Ten genes were expressed differently (false discovery rate, 0.05) in MDA prostate cancer 118b cells with downregulated beta-catenin. One such gene, hyaluronan synthase 2 (HAS2), synthesizes hyaluronan, a core component of the extracellular matrix. We confirmed HAS2 upregulation in PC-3 cells transfected with D32G-mutant beta-catenin. Finally, we found nuclear localization of beta-catenin in 10 of 27 human tissue specimens; this localization was inversely associated with AR expression (P = 0.056, Fisher%26apos;s exact test), suggesting that reduced AR expression enables Wnt/beta-catenin signaling. %26lt;br%26gt;Conclusion: We identified a previously unknown downstream target of beta-catenin, HAS2, in prostate cancer, and found that high beta-catenin nuclear localization and low or no AR expression may define a subpopulation of men with bone metastatic prostate cancer. These findings may guide physicians in managing these patients. Clin Cancer Res; 18(3); 726-36.

• 出版日期2012-2-1