Anticancer activity of bovine alpha-lactalbumin treated with microbial transglutaminase

作者：Jeong Ji Eun; Hong Youn Ho^*

来源：International Journal of Dairy Technology, 2012, 65(2): 212-216.

DOI：10.1111/j.1471-0307.2012.00820.x

• a-Lactalbumin
• Anticancer activity
• Microbial transglutaminase
• Tryptic hydrolysates
• Apoptosis

This study investigated the effects of bovine a-lactalbumin (a-La) treated with microbial transglutaminase on human cancer cells, cell cultures and growth rate assays. The anticancer activity for 10 mg/mL of bovine a-lactalbumin (a-La) was measured as similar to 90% in a human colorectal cancer cell line HCT 116. For the human bone cancer cell line SJSA-1, a-La hydrolysis resulted in higher cytotoxicity compared to untreated tumour cells. The formation of polymers of a-La was suppressed by the addition of ethylenediaminetetraacetic acid, indicating that polymers of a-La are promoted by metal ions such as Ca2+. The effect of a-La on the morphology of SJSA-1 cells was manifested as morphological changes compatible with apoptosis. Bovine milk a-La with and without microbial transglutaminase is considered a valuable food ingredient and a nutraceutical for human health.

• 出版日期2012-5