科研之友
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摘要
Objective: Osteonecrosis of the femoral head (ONFH) frequently results in collapse and subsequent degenerative joint disease of the hip. Bisphosphonates (BPs) therapy has been reported to retard osteoporosis; however, the effect of BPs on the prevention of glucocorticoid-induced ONFH remains controversial. Methods: In the current study, we investigated the state of MC3T3-E1 in vitro, in the presence of dexamethasone (DEX) with or without alendronate (ALN) treatment and detected osteogenesis-associated proteins, namely osteopontin (OPN) and osteocalcin (OCN). In in vivo studies, we investigated the preventive effect of ALN on methylprednisolone (MP)-induced ONFH in rats. We employed micro-CT scanning, angiography, and histologic and immunohistochemical analysis to demonstrate ALN's effect on the subchondral trabeculae of the femoral head. Results: In in vitro study, we observed that high-dose DEX clearly inhibited osteogenic differentiation of MC3T3-E1. OPN and OCN expression levels in MC3T3E1 were downregulated by DEX; however, the suppressive effect could be reserved when ALN was supplemented. The mineralization of MC3T3-E1 was improved significantly in the DEX+ALN group compared to that of DEX solely. In in vivo study, the parameters of micro-structure of trabecular bone were greatly ameliorated by ALN. Moreover, angiography of the femoral head revealed a protective role of ALN on osseous circulation. Combined effects of ALN contributed to decreased incidence of MP-induced ONFH in rats. Conclusion: ALN counteracts the suppressive effect of DEX on MC3T3-E1 cells, which benefits the trabecular bone and microcirculation of the femoral head in rats and can prevent glucocorticoid-induced ONFH.
- 出版日期2017
- 单位上海交通大学
