A novel synthetic method for preparation of some folates

作者：Zhou Minghao; Hong Mei; Xiao Guomin^*

来源：Research on Chemical Intermediates, 2013, 39(5): 2211-2218.

DOI：10.1007/s11164-012-0751-2

• Calcium-5-methyltetrahydrofolate
• Gradient elution
• Reduction
• LC-MS
• 5,6,7,8-Tetrahydrofolic acid

An improved method was developed for preparation of 5,6,7,8-tetrahydrofolic acid (THF) and calcium-5-methyltetrahydrofolate (5-MTHF-Ca) by reduction of folic acid using KBH4 catalyzed by Pb(NO3)(2). The yields of THF and 5-MTHF-Ca were 56.5 and 42.7 %, respectively. A convenient method for measurement of THF and 5-MTHF-Ca using liquid chromatography-mass spectrometry (LC-MS) was also established, enabling analysis of those folates within 10 min without application of gradient elution.

• 出版日期2013-5
• 单位东南大学