Development of microbial community structure in vegetable-growing soils from open-field to plastic-greenhouse cultivation based on the PLFA analysis

作者：Yao, Zhiyuan; Xing, Jiajia; Gu, Haiping; Wang, Haizhen^*; Wu, Jianjun; Xu, Jianming; Brookes, Philip C.

来源：Journal of Soils and Sediments, 2016, 16(8): 2041-2049.

DOI：10.1007/s11368-016-1397-2

• Microbial community
• Phospholipid fatty acids (PLFAs)
• Plastic-greenhouse cultivation
• Shannon-Weaver index

The objectives of this study were to investigate (i) how the changes in cultivation pattern of vegetable affect soil microbial communities and (ii) the relationships between soil physico-chemical properties and microbial community structure. @@@ Soil samples were collected from fields growing vegetable crops with various times of plastic-greenhouse cultivation (0, 1, 4, 7 and 15 years, respectively). Phospholipid fatty acid (PLFA) analysis was conducted to reveal the soil microbial community of the test soils. @@@ The open-field soil had the highest total PLFAs amount. Although the Shannon-Weaver index was also highest in the open-field soil, the difference was not significant. Plastic-greenhouse cultivation decreased PLFAs species diversity and richness. Cluster analysis and principal component analysis (PCA) of the PLFA profiles revealed distinct groupings at different times during plastic-greenhouse cultivation. @@@ Ultimately, PLFA analyses showed that long-term plastic-greenhouse cultivation make the physiological status of soil microbial community worse and increased stress level of microorganisms. And soil microbial community was significantly affected by field water capacity and water-soluble organic carbon. The study highlights the potential risk of long-term plastic-greenhouse cultivation to soil microbial community.

• 出版日期2016-8
• 单位浙江大学