A Hierarchical Combination of Factors Shapes the Genome-wide Topography of Yeast Meiotic Recombination Initiation

作者：Pan Jing; Sasaki Mariko; Kniewel Ryan; Murakami Hajime; Blitzblau Hannah G; Tischfield Sam E; Zhu Xuan; Neale Matthew J; Jasin Maria; Socci Nicholas D; Hochwagen Andreas; Keeney Scott^*

来源：Cell, 2011, 144(5): 719-731.

DOI：10.1016/j.cell.2011.02.009

The nonrandom distribution of meiotic recombination influences patterns of inheritance and genome evolution, but chromosomal features governing this distribution are poorly understood. Formation of the DNA double-strand breaks (DSBs) that initiate recombination results in the accumulation of Spo11 protein covalently bound to small DNA fragments. By sequencing these fragments, we uncover a genome-wide DSB map of unprecedented resolution and sensitivity. We use this map to explore how DSB distribution is influenced by large-scale chromosome structures, chromatin, transcription factors, and local sequence composition. Our analysis offers mechanistic insight into DSB formation and early processing steps, supporting the view that the recombination terrain is molded by combinatorial and hierarchical interaction of factors that work on widely different size scales. This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We also discuss implications for evolutionary dynamics of recombination hot spots.

• 出版日期2011-3-4