A reliable and rapid tool for plasma quantification of 18 psychotropic drugs by ESI tandem mass spectrometry

作者：Vecchione Gennaro; Casetta Bruno; Chiapparino Antonella; BertolinoB Alessandro; Tomaiuolo Michela; Cappucci Filomena; Gatta Raffaella; Margaglione Maurizio; Grandone Elvira^*

来源：Journal of Pharmaceutical and Biomedical Analysis, 2012, 67-68: 104-113.

DOI：10.1016/j.jpba.2012.04.016

• Therapeutic drug monitoring
• Psychotropic drugs
• Poly-pharmacy
• LC-MS/MS
• Protein precipitation
• sMRM

A simple liquid chromatographic tandem mass spectrometry (LC-MS/MS) method has been developed for simultaneous analysis of 17 basic and one acid psychotropic drugs in human plasma. %26lt;br%26gt;The method relies on a protein precipitation step for sample preparation and offers high sensitivity, wide linearity without interferences from endogenous matrix components. %26lt;br%26gt;Chromatography was run on a reversed-phase column with an acetonitrile-H2O mixture. The quantification of target compounds was performed in multiple reaction monitoring (MRM) and by switching the ionization polarity within the analytical run. %26lt;br%26gt;A further sensitivity increase was obtained by implementing the functionality %26quot;scheduled multiple reaction monitoring%26quot; (sMRM) offered by the recent version of the software package managing the instrument. %26lt;br%26gt;The overall injection interval was less than 5.5 min. Regression coefficients of the calibration curves and limits of quantification (LOQ) showed a good coverage of over-therapeutic, therapeutic and sub-therapeutic ranges. Recovery rates, measured as percentage of recovery of spiked plasma samples, were %26gt;= 94%. Precision and accuracy data have been satisfactory for a therapeutic drug monitoring (TDM) service as for managing plasma samples from patients receiving psycho-pharmacological treatment.

• 出版日期2012-9