Ultra fast analysis of subcutaneous pork fat

作者：Ficarra Alessandro^*; Lo Fiego Domenico Pietro; Minelli Giovanna; Antonelli Andrea

来源：Food Chemistry, 2010, 121(3): 809-814.

DOI：10.1016/j.foodchem.2010.01.003

• Fatty acid analysis
• Transesterification method
• Rapid derivatization
• Fast GC
• Transmethylation

Narrow-bore GC columns have led to the resolution of complex matrices in a few minutes. To set up a method able to cope with the velocity of ultra fast gas-chromatographic separation, a comparison among four of the most popular lipid transmethylation was applied to pork fat. Different catalysts for transmethylation were tested (NaOCH(3), BF(3), H(2)SO(4), and KOH), and were compared for yield and rate of transmethylation, cost, and safety.
In all cases, KOH gave the best performance of all the considered parameters. For this reason, it is particularly suitable when a large number of samples are being analysed. Although it is very effective in the transesterification of neutral lipids, it does not tolerate water and is unsuitable for free fatty acid derivatization.

• 出版日期2010-8-1