Protein Profiling the Differences Between Diabetic and Normal Mouse Cumulus Cells

作者：Jiang, Guang-Jian; Ma, Jun-Yu; Zhang, Guang-Li; Mo, Fang-Fang; Zhang, Dong-Wei; Gao, Si-Hua^*; Li, Xiu-Li

来源：Molecular Reproduction and Development, 2014, 81(12): 1080-1085.

DOI：10.1002/mrd.22419

• DNA METHYLATION
• GLUCOSE
• OOCYTES
• FERTILIZATION
• METABOLISM
• EXPRESSION
• MATURATION
• OVIDUCT
• GENES

As the number of young people suffering from diabetes increases worldwide, the impact of this disease on human reproduction urgently needs to be addressed. Here we compared the proteomes of cumulus cells of super-ovulated cumulus-oocyte complexes from diabetic and normal mice. We identified 57 up-regulated and 74 down-regulated proteins in diabetic cumulus cells; among these groups were proteins associated with cell cycle, cellular communication, epigenetic regulation, protein localization, and chromatin organization all in accordance with type I diabetes. The poor-quality follicles derived from diabetic mice were further enforced by the presence of glycoproteins that are specifically expressed by the oocyte or oviductal epithelial cells in the cumulus-cell samples. In conclusion, the proteomic differences between diabetic and normal cumulus cells provide targets for improving the reproduction health of type I diabetic patients. Mol. Reprod. Dev. 81: 1080-1085, 2014.

• 出版日期2014-12
• 单位中国人民解放军总医院; 北京中医药大学; 青岛农业大学