Larsenia salina gen. nov., sp nov., a new member of the family Halomonadaceae based on multilocus sequence analysis

作者：Jose Leon Maria; Sanchez Porro Cristina; de la Haba Rafael R; Llamas Inmaculada; Ventosa Antonio^*

来源：Systematic and Applied Microbiology, 2014, 37(7): 480-487.

DOI：10.1016/j.syapm.2014.07.006

• Moderately halophilic bacteria
• Larsenia
• Larsenia salina
• Gammaproteobacteria
• Hypersaline environments
• Salterns

Two Gram-staining-negative, moderately halophilic bacteria, strains M1-18(T) and L1-16, were isolated from a saltern located in Huelva (Spain). They were motile, strictly aerobic rods, growing in the presence of 3-25% (w/v) NaCl (optimal growth at 7.5-10% [w/v] NaCl), between pH 4.0 and 9.0 (optimal at pH 6.0-7.0) and at temperatures between 15 and 40 degrees C (optimal at 37 degrees C). Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that both strains showed the higher similarity values with Chromohalobacter israelensis ATCC 43985(T) (95.2-94.8%) and Chromohalobacter salexigens DSM 3043(T) (95.0-94.9%), and similarity values lower than 94.6% with other species of the genera Chromohalobacter, Kushneria, Cobetia or Halomonas. Multilocus sequence analysis (MLSA) based on the partial sequences of atpA, rpoD and secA housekeeping genes indicated that the new isolates formed an independent and monophyletic branch that was related to the peripheral genera of the family Halomonadaceae, Halotalea, Carnimonas and Zymobacter, supporting their placement as a new genus of the Halomonadaceae. The DNA-DNA hybridization between both strains was 82%, whereas the values between strain M1-18(T) and the most closely related species of Chromohalobacter and Kushneria were equal or lower to 48%. The major cellular fatty acids were C-18:1 omega 7c/C-18:1 omega 6c, C-16:0, and C-16:0 omega 7c/C-16:1 omega 6c, a profile that differentiate this new taxon from species of the related genera. We propose the placement of both strains as a novel genus and species, within the family Halomonadaceae, with the name Larsenia sauna gen. nov., sp. nov. The type strain is M1-18(T) (= CCM 8464 = CECT 8192(T) = IBRC-M 10767(T) = LMG 27461(T)).

• 出版日期2014-10