Aspects of trapping efficiency and matrix effects in the development of a restricted-access-media-based trap-and-elute liquid chromatography with mass spectrometry method

作者：Papouskova Barbora; Fan Hui; Lemr Karel; Schug Kevin A^*

来源：Journal of Separation Science, 2014, 37(16): 2192-2199.

DOI：10.1002/jssc.201400369

• Biological fluids
• Bulk derivatization
• Estradiol quantification
• Estrogens
• On-line sample preparation

Online restricted access media with liquid chromatography and tandem mass spectrometry for the direct analysis of small molecules in biological fluids represents an interesting alternative to time-demanding traditional sample preparation techniques. In this study, important considerations concerning the development of a restricted access media with liquid chromatography and tandem mass spectrometry method for the analysis of dansylated estrogens in biological matrix are presented. Parameters influencing peak tailing and trapping efficiency were evaluated. The key factors included the ion strength of the mobile phase, a loading flow rate of the sample onto the trap column, and selection of a proper stationary phase of the trap column for a given set of analytes. These parameters have proven to be essential for minimizing any unwanted chromatographic peak tailing. The bulk derivatization of the analytes in the biological fluids and its relationship to the observed matrix effects was evaluated as well.

• 出版日期2014-8