Expression of platelet derived growth factor beta receptor, its activation and downstream signals in bovine cutaneous fibropapillomas

作者：Bocaneti F; Altamura G; Corteggio A; Martano M; Roperto F; Velescu E; Borzacchiello G^*

来源：Research in Veterinary Science, 2013, 94(3): 596-601.

DOI：10.1016/j.rvsc.2012.10.011

• Bovine papillomavirus
• E5
• PDGF beta R

Bovine cutaneous fibropapillomas are benign skin tumours formed by proliferation of epidermal keratinocytes and dermal fibroblasts caused by bovine papillomaviruses (BPVs). BPV E5 oncoprotein plays a key role in neoplastic cell transformation by specifically binding to the platelet derived growth factor beta receptor (PDGF beta R) causing its phosphorylation and activation of proliferation and survival signal transduction pathways, among these phosphatidyl inositol-3-kinase (PI3K)/Akt and Ras-mitogen-activated-protein-kinase-Erk (Ras-MAPK-Erk) pathways. The aim of this study was to investigate the expression of PDGF beta R, its phosphorylation status and expression of the downstream molecules phospho-Akt (pAkt) and phospho-Erk (pErk), in naturally occurring bovine cutaneous fibropapillomas. By immunohistochemistry on serial sections we showed cytoplasmic co-expression of the PDGF beta R and E5 protein in neoplastic tissue. Western blot analysis revealed that PDGF beta R was phosphorylated in higher amount in tumour samples compared to normal skin. pAkt, but not pErk, was also overexpressed in tumour samples. These findings may provide new insights into the aetiopathogenic mechanisms underlying naturally occurring bovine fibropapillomas and contribute to understanding the molecular scenario underlying BPV induced tumourigenesis.

• 出版日期2013-6