Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability

作者：Zhou HM; Jia L; Wang SJ; Wang HM; Chu HY; Hu YC; Cao J; Zhang JN^*

来源：Biochemical and Biophysical Research Communications, 2006, 345(1): 486-494.

DOI：10.1016/j.bbrc.2006.03.246

• caveolin-1
• hepatocarcinoma
• lymphatic metastasis
• VEGFA

Caveolin-1 is the major component protein of caveolae and associated with a lot of cellular events such as endocytosis, cholesterol homeostasis, signal transduction, and tumorigenesis. The majority of results suggest that caveolin-1 might not only act as a tumor suppressor gene but also a promoting metastasis gene. In this study, the divergent expression and roles of caveolin-1 were investigated in mouse hepatocarcinoma cell lines Hca-F, Hca-P, and Hepal-6, which have high, low, and no metastatic potential in the lymph nodes, as compared with normal mouse liver cell line IAR-20. The results showed that expression of caveolin-1 mRNA and protein along with the amount of caveolae number in Hca-F cells was higher than that in Hca-P cells, but was not detectable in Hepal-6 cells. When caveolin-1 expression in Hca-F cells was down-regulated by RNAi approach, Hca-F cells proliferation rate in vitro declined and the expression of lymphangiogenic factor VEGFA in Hca-F decreased as well. Furthermore, in vivo implantation assay indicated that reduction of caveolin-1 expression in Hca-F prevented the lymphatic metastasis tumor burden of Hca-F cells in 615 mice. These results suggest that caveolin-1 facilities the lymphatic metastasis ability of mouse hepatocarcinoma cells via regulation tumor cell growth and VEGFA expression.

• 出版日期2006-6-23
• 单位大连医科大学; 辽东学院