A bisected pupil for studying single-molecule orientational dynamics and its application to three-dimensional super-resolution microscopy

作者：Backer Adam S^*; Backlund Mikael P; von Diezmann Alexander R; Sahl Steffen J; Moerner W E

来源：Applied Physics Letters, 2014, 104(19): 193701.

DOI：10.1063/1.4876440

A phase mask design that we term a "bisected pupil" (BSP) provides several advantages for single-molecule optical imaging. When using the BSP with a dual-polarization optical Fourier processing system, both the position and dipole orientation of individual fluorescent molecules may be measured from a single camera image. In the context of single-molecule super-resolution microscopy, this technique permits one to diagnose, and subsequently to remove imaging artifacts resulting from orientation-induced localization errors. If the molecules labeling a structure are rotationally mobile, thus mitigating dipole orientation errors, this technique enables super-resolution imaging in three dimensions. We present simulations and experimental verification.

• 出版日期2014-5-12