A BACTERIAL, NITRIC-OXIDE SYNTHASE FROM A NOCARDIA SPECIES

作者：CHEN YJ; ROSAZZA JPN

来源：Biochemical and Biophysical Research Communications, 1994, 203(2): 1251-1258.

DOI：10.1006/bbrc.1994.2317

Nitric oxide synthase (EC 1.14.23) was discovered in a Nocardia species. The bacterial nitric oxide synthase was purified as much as 380 fold by affinity chromatography over 2',5'-ADP-agarose. The partially purified enzyme required NADPH, O-2, Ca++, FAD, FMN, and tetrahydrobiopterin as cofactors in the conversion of L-arginine to L-citrulline and nitric oxide. The apparent Km for L-arginine was determined to be 8.2 mu M, and the Vmax was 840 nmole NADPH consumed/min/mg protein. The enzyme was competetively inhibited by N-G-nitro-arginine with an apparent Ki of 14.6 mu M. The experimental evidence provides confirmation of the first microbial nitric oxide synthase in microorganisms.

• 出版日期1994-9-15