Acaricidal Activity of DHEMH, Derived from Patchouli Oil, against House Dust Mite, Dermatophagoides farinae

作者：Wu, Hai Qiang^*; Li, Li; Li, Jing; He, Zhen Dan; Liu, Zhi Gang; Zeng, Qing Qian; Wang, Yu Sheng

来源：CHEMICAL & PHARMACEUTICAL BULLETIN, 2012, 60(2): 178-182.

DOI：10.1248/cpb.60.178

• natural acaricide
• Dermatophagoides farinae
• Pogostemon cablin (Blanco)
• identification
• action mode

This study characterized the acaricidal activity of constituents of patchouli oil extracted from (Pogostemon cablin (Blanco) BENTH) against the house dust mite, Dermatophagoides farinae. A new compound, 2-(1,3-dihydroxy-but-2-enylidene)-6-methyl-3-ozo-heptanoic acid (DHEMH), was isolated from patchouli oil and characterized by H-1-NMR, C-13-NMR, LC-MS and elemental analysis (EA). This active component was identified as the hydrolysate of pogostone. Fifteen other constituents found in patchouli oil were also identified by GC-MS, including patchouli alcohol and pogostone. LD50 studies carried out over 24h using contact toxicity tests identified DHEMH as the most toxic compound to D. farinae (2.04 mu g/cm(2)), followed by patchouli oil (6.11 mu g/cm(2)), benzyl benzoate (BP) (9.31 mu g/cm(2)) and dibutyl phthalate (DBP) (58.52 mu g/cm(2)). In vapor phase toxicity tests, all of these compounds were more effective in closed than open containers, indicating that the most efficient mode of delivery for these compounds is the vapor phase. These results indicate that DHEMH and patchouli oil merit further study as potential agents for the control of D. farinae.

• 出版日期2012-2
• 单位广东省中医研究所; 深圳大学