Toll-like receptor 3 agonist enhances IFN-gamma and TNF-alpha production by murine uterine NK cells

作者：Zhang Jianhong; Sun Rui; Wei Haiming^*; Wu Dongmei; Tian Zhigang

来源：International Immunopharmacology, 2007, 7(5): 588-596.

DOI：10.1016/j.intimp.2006.12.014

• polyinosinic-polycytidylic acid
• uterine natural killer cells
• IFN-gamma
• TNF-alpha

To understand the response of murine uterine natural killer (uNK) cells to Toll-like receptor (TLR) 3 agonist at the early gestation stage, CBA x DBA/2 mice were intraperitoneally (i.p.) injected with polyinosinic-polycytidylie acid (poly 1:Q, the specific TLR3 agonist, at a dose of 10 mu g/g BW or PBS at gestation day (gd) 6.5. The CD69 expression of uNK (DX5(+)CD(3-)) cells was highly up-regulated and reached 92.3 0.9%, the percentage of intracellular TNF-alpha(+) or IFN-gamma(+) uNK (DX5(+)CD3(-)) cells in the implantation sites of CBA x DBA/2 matings was also significantly increased 24 h after poly I:C injection. Surprisingly, poly I:C treatment significantly decreased the total number of uNK cells (either DX5+CD3- or DBA(+)) at fetal-maternal surface, but had no influence on local NKT cells, T cells and DCs. This investigation will help to explain the central role for hyperactivated uNK cells in the progress of mice pregnancy.

• 出版日期2007-5
• 单位中国科学技术大学