Mitochondrial and nuclear markers for the authentication of partridge meat and the specific identification of red-legged partridge meat products by polymerase chain reaction

作者：Rojas M; Gonzalez I; Pavon M A; Pegels N; Hernandez P E; Garcia T; Martin R^*

来源：Poultry Science, 2011, 90(1): 211-222.

DOI：10.3382/ps.2010-00895

• 12S ribosomal ribonucleic acid gene
• rhodopsin gene
• polymerase chain reaction
• partridge meat
• red-legged partridge

Two PCR assays for the identification of partridge meat (red-legged partridge, chukar partridge, barbary partridge, and gray partridge species) and the specific identification of red-legged partridge meat products were developed based on species-specific primers targeting the 12S ribosomal RNA mitochondrial gene. Moreover, various PCR techniques based on the use of random amplified polymorphic DNA markers and nuclear growth hormone and rhodopsin genes were tested to find a method for the differentiation between pure and hybrid red-legged partridges. Among these techniques, the PCR method based on the amplification and sequencing of a nuclear rhodopsin gene fragment was selected as a suitable tool for the discrimination among meats from pure and hybrid red-legged partridge individuals. The PCR assays reported in this work could be useful in inspection programs to verify the correct labeling of raw and heat-treated partridge meat products.

• 出版日期2011-1-1