Activation of polymorphonuclear leukocytes (basophils, neutrophils and eosinophils) by food allergens in IgE-dependant food allergy in children

作者：Masson A^*; Menetrey C; Bahans C; St Laudy J

来源：Revue Francaise d'Allergologie, 2013, 53(8): 615-623.

DOI：10.1016/j.reval.2013.08.002

• Food allergy
• Children
• Basophils
• Neutrophils
• Eosinophils
• CD11b
• CD63
• Leukocyte activation
• Flow cytometry

Aim of the study. - To analyze the differential expression of activation markers in basophils, neutrophils and eosinophils in a pediatric population with clinical and laboratory evidence of IgE-dependent food allergy.
Methods. - Cell activation by the most common food allergens (peanut, milk, egg, hazel nuts and shrimp) was analyzed by flow cytometry (FC) with multiple markers: CD63 and CD11b for basophils and eosinophils and CD11b for neutrophils.
Results. - Fifty-five of the 79 children included in the study had a positive FC result for at least one food allergen. The activation marker CD11b was expressed more often in all three of the polymorphonuclear cells (p = 0.0036). This marker was found more often in the eczema group than in the rash group (p = 0.01). For a threshold of 12% CD63 positivity, there was a significant relationship (p < 0.001) between the oral food challenge (OFC) test results and the expression Of CD63 by basophils. The expression of CD11b was more frequent in subjects with a negative OFC (p < 0.02).
Conclusion. - CD11b integrin had not been investigated previously as a possible allergen-dependent activation marker for polymorphonuclear leukocytes. Our study showed that the expression of CD11b by eosinophils and neutrophils may be the result of activation by allergen, and that its IgE dependence was confirmed by overexpression of this marker. The inverse relationship between the expression of CD63 and CD11b in urticaria and atopic dermatitis suggests that they may be involved in the migration of leukocytes, basophils in particular, into affected tissues.

• 出版日期2013-12