Anti-inflammatory Activity of Melampyrum barbatum and Isolation of Iridoid and Flavonoid Compounds

作者：Haznagy-Radnai Erzsebet; Fasi Laura; Weber Edit; Pinke Gyula; Kiraly Gergely; Sztojkov-Ivanov Anita; Gaspar Robert; Hohmann Judit^*

来源：Natural Product Communications, 2018, 13(3): 235-236.

DOI：10.1177/1934578x1801300301

• Melampyrum barbatum
• Scrophulariaceae
• Iridoids
• Flavonoids
• Anti-inflammatory activity

Melampyrum barbatum Waldst. & Kit. ex Willd. (Scrophulariaceae) has been used in traditional medicine for the treatment of rheumatic complaints and different skin diseases. In the course of our study the anti-inflammatory activity of the aerial parts of M barbatum was evaluated. A MeOH extract was prepared and consecutively partitioned with CHCl3, EtOAc and n-BuOH. The fractions were assayed in in vivo carrageenan-induced rat paw oedema model. The intraperitoneally administered n-BuOH phase exerted marked inhibitory effect (33.6 %, p < 0.01). Multistep chromatographic separation afforded mussaenoside and aucubine from n-BuOH fraction. Moreover, 8-epiloganin, loganic acid and mussaenoside were obtained from EtOAc fraction and apigenin, luteolin, benzoic acid and galactitol from CHCl3 fraction. These data validate the ethnomedicinal use of M barbatum for the treatment of inflammatory diseases and reveal that iridoids and flavonoids could be responsible for the anti-inflammatory effect of this species.

• 出版日期2018-3