A new insertion sequence, IS231M, in an autoagglutinable isolate of Bacillus thuringiensis

作者：Jung YC; Xu D; Chung YS; Cote JC^*

来源：Plasmid, 2001, 45(2): 114-121.

DOI：10.1006/plas.2000.1508

• Bacillus thuringiensis
• insertion sequence
• IS231M

An insertion sequence was isolated from an autoagglutinable strain of Bacillus thuringiensis. Analysis of its DNA sequence revealed high homology to the IS231 family. The name IS231M is proposed for this new insertion sequence. IS231M is 1652 bp long and is delimited by two imperfect 20bp inverted repeat sequences with two mismatches, which are flanked by two perfect I I-bp direct repeats (DRs). The region upstream of the open reading frame, presumed to be able to form a stable hairpin structure, is particularly well conserved in IS231M. Based on primary nucleotide sequences, IS231M is most homologous to IS231F and IS231G and most distant from IS231V and IS231W. However, as opposed to the single transposase A ORF found in IS131A. -B. -C. -D. -E and -G. IS231M has two overlapping open reading frames, OFR1 and ORF2, that could code for polypeptides of 334 and 143 amino acids, respectively Whether IS231M is a functional transposable element remains to be determined.

• 出版日期2001-3