Compounds having thiourea moiety as derivatization reagents in liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS): synthesis of derivatization reagents for carboxylic acids

作者：Inoda Hirotaka; Nishiyama Taihei; Yoshikado Takashi; Suwanai Yusuke; Santa Tomofumi^*

来源：Biomedical Chromatography, 2011, 25(6): 635-640.

DOI：10.1002/bmc.1501

• liquid chromatography/electrospray ionization-tandem mass spectrometry
• LC/ESI-MS/MS
• derivatization reagent
• carboxylic acid

The derivatization reagents for carboxylic acids, N-(Pyridin-3-yl)hydrazinecarbothioamide, N-[4-(dimethylamino)phenyl]hydrazinecarbothioamide, 1-(2-aminoethyl)-3-(pyridin-3-yl)thiourea, 1-(2-aminoethyl)-3-[4-(dimethylamino)phenyl]thiourea and 4-(2-aminoethyl)-N-phenylpiperazine-1-carbothioamide were synthesized. These reagents reacted with carboxylic acids at 60 degrees C for 45 min in the presence of the condensation reagents. The generated derivatives were favorably separated on the reversed-phase column and sensitively detected by electrospray ionization tandem mass spectrometry. These reagents enhanced the electrospray ionization response of the analyte and generated a particular product ion efficiently by collision-induced dissociation, and thus they were suitable for MS/MS detection.

• 出版日期2011-6