科研之友
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摘要
A simple and quick method is described for the determination of ligustrazine in rhizomes of Ligusticum chuanxiong. The ligustrazine in the sample was extracted using methanol and analyzed by reversed-phase high performance liquid chromatography. Chromatography separation was performed using Agilent 1100 series HPLC system with a waters symmetry C-18 column and isocratic elution with a mixture of three solvents: acetonitrile, methanol and 1 % aqueous acetic acid (20: 40: 40, v/v/v). The effluent was monitored using a VWD detector set at 294 nm. The average recoveries were 84.93 -88.65 % (n = 3). The limits of detection and quantification were 0.044 and 0.133 mu g mL(-1), respectively. The method has been successfully applied to the analysis of L. chuanxiong samples. For 37 samples, the ligustrazine content varied from 0.22 to 1.06 mg g(-1).
- 出版日期2014-8
- 单位四川农业大学
