Artemisia fukudo essential oil attenuates LPS-induced inflammation by suppressing NF-kappa B and MAPK activation in RAW 264.7 macrophages

作者：Yoon W J; Moon J Y; Song G; Lee Y K; Han M S; Lee J S; Ihm B S; Lee W J; Lee N H; Hyun C G^*

来源：Food and Chemical Toxicology, 2010, 48(5): 1222-1229.

DOI：10.1016/j.fct.2010.02.014

• Artemisia fukudo
• Chemical composition
• Essential oil
• Inflammation
• Nuclear factor-kappa B

In the present study, the chemical constituents of Artemisia fukudo essential oil (AFE) were investigated using GC-MS. The major constituents were alpha-thujone (48.28%), beta-thujone (12.69%), camphor (6.95%) and caryophyllene (6.01%). We also examined the effects of AFE on the production of nitric oxide (NO), prostaglandin E-2 (PGE(2)), tumour necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and IL-6, in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. Western blotting and RT-PCR tests indicated that AFE has potent dose-dependent inhibitory effects on pro-inflammatory cytokines and mediators. We investigated the mechanism by which AFE inhibits NO and PGE2 by examining the level of nuclear factor-kappa B (NF-kappa B) activation within the mitogen-activated protein kinase (MAPK) pathway, which is an inflammation-induced signal pathway in RAW 264.7 cells. AFE inhibited LPS-induced ERK, JNK, and p38 phosphorylation. Furthermore, AFE inhibited the LPS-induced phosphorylation and degradation of I kappa-B-alpha, which is required for the nuclear translocations of the p50 and p65 NF-kappa B subunits in RAW 264.7 cells. Our results suggest that AFE might exert an anti-inflammatory effect by inhibiting the expression of pro-inflammatory cytokines. Such an effect is mediated by a blocking of NF-kappa B activation which consequently inhibits the generation of inflammatory mediators in RAW264.7 cells. AFE may be useful for treating inflammatory diseases.

• 出版日期2010-5