Detection of avian-like rotavirus A VP4 from a calf in Japan

作者：Mitake Hiromichi; Ito Naoto; Okadera Kota; Okada Kazuma; Nakagawa Kento; Tanaka Tomomi; Katsuragi Kiyohito; Kasahara Kasumi; Nihongi Toshihide; Tsunemitsu Hiroshi; Sugiyama Makoto^*

来源：Journal of Veterinary Medical Science, 2015, 77(2): 221-224.

DOI：10.1292/jvms.14-0379

• avian-like
• cattle
• rotavirus A
• VP4

A total of 568 normal feces from calves on a beef farm in Fukui Prefecture, Japan, in 2011-2012 were examined by RT-semi-nested PCR for rotavirus A (RVA) VP4 genes. Through partial sequencing and BLAST analyses of 84 VP4-positive specimens, we identified an avian-like RVA strain, N2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. Phylogenetic analysis also revealed a close genetic relationship between N2342 and avian RVAs, suggesting bird-to-cattle transmission. We observed frequent contact of wild birds with calves in the farm, suggesting that these birds were the source of the virus.

• 出版日期2015-2