Assessment of sensory metabolites distribution in 3 cactus Opuntia ficus-indica fruit cultivars using UV fingerprinting and GC/MS profiling techniques

作者：Farag Mohamed A; Maamoun Amal A; Ehrlich Anja; Fahmy Sherifa; Wesjohann Ludger A

来源：LEBENSMITTEL-WISSENSCHAFT UND-TECHNOLOGIE-FOOD SCIENCE AND TECHNOLOGY, 2017, 80: 145-154.

DOI：10.1016/j.lwt.2017.02.014

• Chemometrics
• Opuntia ficus-indica
• SPME
• Sugars
• Volatiles

Among most propagated and worldwide cacti used for commercial (food) production is Opuntia ficus-indica. The present study aimed at investigating aroma compound and metabolites distribution in cactus fruits from 3 cultivars (cvs): red 'Rose', yellow-orange 'Gialla' and greenish-white 'Bianca' represented by both its pulp and skin samples. Two methods were applied including UV-vis fingerprinting versus gas chromatography coupled to mass spectrometry (GC-MS). Betalains predominated in red fruits, whereas carotenoids and chlorophyll were more abundant in orange and green fruits, respectively, as revealed from their crude extracts UV absorption spectra. Volatiles were profiled using headspace solid-phase micro-extraction (SPME) coupled to GC-MS. 40 Volatiles were identified with short chain aldehydes (25-32%) and acids (25-29%) as the major volatile classes. Cultivars exhibited comparable aroma profiles suggesting that volatiles cannot serve as a chemical fingerprint to distinguish between cvs. Primary metabolites mediating for fruit taste and nutritional value viz. sugars and amino acid were profiled using GC-MS post silylation with 82 identified metabolites. Glucose (62%) and fructose (16%) were found to predominate sugar composition, whereas proline was the major amino acid (3-8%). Multivariate data analyses revealed for betalain and disaccharides enrichment i.e., turanose and sucrose in fruit skin versus proline, talopyranose and lyxopyranose abundance in pulp tissue.

• 出版日期2017-7