Multicompartment Lipid Cubic Nanoparticles with High Protein Upload: Millisecond Dynamics of Formation

作者：Angelov Borislav; Angelova Angelina^*; Filippov Sergey K; Drechsler Markus; Stepanek Petr; Lesieur Sylviane

来源：ACS Nano, 2014, 8(5): 5216-5226.

DOI：10.1021/nn5012946

• lipid-protein nanoassembly
• dynamic membrane curvature
• amphiphile nanoarchitectonics
• multicompartment lipid nanoparticle
• morphological transition pathway
• millisecond time-resolved SAXS
• cryo-TEM
• BDNF-loaded cubosome

Membrane shapes, produced by dynamically assembled lipid/protein architectures, are crucial for both physiological functions and the design of therapeutic nanotechnologies. Here we Investigate the dynamics of lipid membrane-neurotrophic BDNF protein complexes formation and ordering In nanoparticles, with the purpose of innovation in nanostructure-based neuroprotection and biomimetic nanoarchitectonics. The kinetic pathway of membrane states associated with rapidly occurring nonequilibrium self-assembled lipid/protein nanoarchitectures was determined by millisecond time-resolved small-angle X-ray scattering (SAXS) at high resolution. The neurotrophin binding and millisecond trafficking along the flexible membranes induced an unusual overlay of channel-network architectures including two coexisting cubic lattices epitaxially connected to lamellar membrane stacks. These time-resolved membrane processes, involving intercalation of discrete stiff proteins in continuous soft membranes, evidence stepwise curvature control mechanisms. The obtained three-phase liquid-crystalline nanoparticles of neurotrophic composition put forward important advancements in multicompartment soft-matter nanostructure design.

• 出版日期2014-5