Assessment of Blood Hemodynamics by USPIO-Induced R (1) Changes in MRI of Murine Colon Carcinoma

作者：Gambarota Giulio^*; van Laarhoven H W M; Philippens M; Peeters W J M; Rijken P; van der Kogel A; Punt C J A; Heerschap A

来源：Applied Magnetic Resonance, 2010, 38(3): 349-360.

DOI：10.1007/s00723-010-0124-1

The objective of this study is to assess whether ultrasmall superparamagnetic iron oxide (USPIO)-induced changes of the water proton longitudinal relaxation rate (R (1)) provide a means to assess blood hemodynamics of tumors. Two types of murine colon tumors (C26a and C38) were investigated prior to and following administration of USPIO blood-pool contrast agent with fast R (1) measurements. In a subpopulation of mice, R (1) was measured following administration of hydralazine, a well-known blood hemodynamic modifier. USPIO-induced R (1) increase in C38 tumors (Delta R (1) = 0.072 +/- A 0.0081 s(-1)) was significantly larger than in C26a tumors (Delta R (1) = 0.032 +/- A 0.0018 s(-1), N = 9, t test, P < 0.001). This was in agreement with the immunohistochemical data that showed higher values of relative vascular area (RVA) in C38 tumors than in C26a tumors (RVA = 0.059 +/- A 0.015 vs. 0.020 +/- A 0.011; P < 0.05). Following administration of hydralazine, a decrease in R (1) value was observed. This was consistent with the vasoconstriction induced by the steal effect mechanism. In conclusion, R (1) changes induced by USPIO are sensitive to tumor vascular morphology and to blood hemodynamics. Thus, R (1) measurements following USPIO administration can give novel insight into the effects of blood hemodynamic modifiers, non-invasively and with a high temporal resolution.

• 出版日期2010-6