Differential phosphorylation and n-terminal configuration of capsid subunits in parvovirus assembly and viral trafficking

作者：Gil Ranedo Jon; Hernando Eva; Valle Noelia; Riolobos Laura; Maroto Beatriz; Almendral Jose M^*

来源：Virology, 2018, 518: 184-194.

DOI：10.1016/j.virol.2018.02.018

• Parvovirus
• Capsid phosphorylation
• Nt configuration
• Assembly
• Maturation
• Viral trafficking

The T1 parvovirus Minute Virus of Mice (MVM) was used to study the roles that phosphorylation and N-terminal domains (Nt) configuration of capsid subunits may play in icosahedral nuclear viruses assembly. In synchronous MVM infection, capsid subunits newly assembled as two types of cytoplasmic trimeric intermediates (3VP2, and 1VP1:2VP2) harbored a VP1 phosphorylation level fivefold higher than that of VP2, and hidden Nt. Upon nuclear translocation at S phase, VP1-Nt became exposed in the heterotrimer and subsequent subviral assembly intermediates. Empty capsid subunits showed a phosphorylation level restored to VP1:VP2 stoichiometry, and the Nt concealed in their interior. However ssDNA-filled virus maturing at S/G2 lacked VP1 phosphorylation and one major VP2 phosphopeptide, and exposed VP2-Nt. Endosomal VP2-Nt cleavage resulted in VP3 subunits devoid of any phospholabel, implying that incoming viral particles specifically harbor a low phosphorylation status. Phosphorylation provides a mechanistic coupling of parvovirus nuclear assembly to the cell cycle.

• 出版日期2018-5