A hydrodynamic microchip for formation of continuous cell chains

作者：Khoshmanesh Khashayar^*; Zhang Wei; Tang Shi Yang; Nasabi Mahyar; Soffe Rebecca; Tovar Lopez Francisco J; Rajadas Jayakumar; Mitchell Arnan

来源：Applied Physics Letters, 2014, 104(20): 203701.

DOI：10.1063/1.4879457

Here, we demonstrate the unique features of a hydrodynamic based microchip for creating continuous chains of model yeast cells. The system consists of a disk shaped microfluidic structure, containing narrow orifices that connect the main channel to an array of spoke channels. Negative pressure provided by a syringe pump draws fluid from the main channel through the narrow orifices. After cleaning process, a thin layer of water is left between the glass substrate and the polydimethylsiloxane microchip, enabling leakage beneath the channel walls. A mechanical clamp is used to adjust the operation of the microchip. Relaxing the clamp allows leakage of liquid beneath the walls in a controllable fashion, leading to formation of a long cell chain evenly distributed along the channel wall. The unique features of the microchip are demonstrated by creating long chains of yeast cells and model 15 mu m polystyrene particles along the side wall and analysing the hydrogen peroxide induced death of patterned cells.

• 出版日期2014-5-19