Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing

作者：Bjorn Mortensen K^*; Zallet J; Lillebaek T; Andersen A B; Niemann S; Rasmussen E M; Kohl T A

来源：Journal of Clinical Microbiology, 2015, 53(8): 2716-2719.

DOI：10.1128/JCM.00662-15

Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.

• 出版日期2015-8