A Facile Method for the Detection of Aldehydes in Oxidized Lipids Using Solid-Phase Microextraction Fiber and Gas Chromatograph Equipped with a Septum-Free Injector

作者：Arato Shingo; Ito Hiroshi; Miyashita Kazuo; Hayakawa Kazuichi; Itabashi Yutaka^*

来源：Journal of Oleo Science, 2009, 58(1): 17-22.

DOI：10.5650/jos.58.17

• septum-free injector
• gas chromatography
• headspace solid-phase microextraction
• oxidized fish oil
• aldehyde

A facile method is described for the detection of volatile odor components from oxidized lipids. The method is based on headspace solid-phase microextraction (HS-SPME) of the compounds in the vial head space and the subsequent analysis of the volatiles by gas chromatography (GC) using a septum-free sample injector. The extraction was done using a 85 mu m carboxen-poly(dimethylsiloxane) SPME fiber and the volatiles were desorbed onto a GC instrument with the septum-free injection port. The septum free injector made it possible to analyze the samples without using a conventional silicone rubber septum, that is easily damaged by the repetitive stubs of a relatively thick blunt needle into the septum and a long desorption time after injection. The method was standardized with authentic C(3)-C(10) aldehydes and was applied to the detection of aldehyde components from oxidized fish oil. Several volatiles, including propanal and hexanal, were clearly separated on a polar capillary column (60 m) with reproducible retention times. The present study demonstrates that the method using the GC septum-free injector and the HS-SPME fiber would be useful for the determination of volatile components derived from edible oils.

• 出版日期2009-1
• 单位河北医科大学