Development of a general-purpose method for cell purification using Cre/loxP-mediated recombination

作者：Kuroki Shunsuke; Akiyoshi Mika; Ideguchi Ko; Kitano Satsuki; Miyachi Hitoshi; Hirose Michiko; Mise Nathan; Abe Kuniya; Ogura Atsuo; Tachibana Makoto^*

来源：GENESIS, 2015, 53(6): 387-393.

DOI：10.1002/dvg.22863

• immunological cell purification
• hCD271
• Cre
• loxP-mediated recombination
• mouse

A mammalian body is composed of more than 200 different types of cells. The purification of a certain cell type from tissues/organs enables a wide variety of studies. One popular cell purification method is immunological isolation, using antibodies against specific cell surface antigens. However, this is not a general-purpose method, since suitable antigens have not been found in certain cell types, including embryonic gonadal somatic cells and Sertoli cells. To address this issue, we established a knock-in mouse line, named R26 KI, designed to express the human cell surface antigen hCD271 through Cre/loxP-mediated recombination. First, we used the R26 Kl mouse line to purify embryonic gonadal somatic cells. Gonadal somatic cells were purified from the R26 KI; Nr5a1-Cre-transgenic (tg) embryos almost equally as efficiently as from Nr5a1-hCD271-tg embryos. Second, we used the R26 KI mouse line to purify Sertoli cells successfully from R26 KI; Amh-Cre-tg testes. In summary, we propose that the R26 KI mouse line is a powerful tool for the purification of various cell types. genesis 53:387-393, 2015.

• 出版日期2015-6
• 单位RIKEN