Analysis of the Roles of Glucose Transporter 1 and Hexokinase 2 in the Metabolism of Glucose by Extrahepatic Bile Duct Cancer Cells

作者：Yoon Sun Och; Jeon Tae Joo^*; Park Joon Seong; Ryu Yong Hoon; Lee Jae Hoon; Yoo Jung Sik; Kim Jae Keun; Yoon Dong Sup; Oh Eun Ji

来源：Clinical Nuclear Medicine, 2015, 40(3): E178-E182.

DOI：10.1097/rlu.0000000000000640

• F-18-FDG
• cholangiocarcinoma
• glucose transporter
• hexokinase

Purpose of the Report: Extrahepatic bile duct (EHD) cancer varies in uptake of FDG. The aim of the present study was to determine the role of glucose transporter (GLUT) 1 and hexokinase (HK) 2 in the glucose metabolism of EHD cancer cells using immunohistochemistry and F-18-FDG PET/CT.
Methods: Twenty-six patients with EHD cancer who underwent baseline PET/CT and surgery were studied. Biopsies were immunohistochemically analyzed using antibodies against GLUT1 and HK2, and the expression was scored from 0 to 4 according to the percentage of stained cells. SUV and tumor-to-liver ratio (T/L-ratio) were obtained from F-18-FDG PET/CT data. SUV and T/L-ratio and GLUT1 and HK2 expression were compared with histological grades and tumor locations (proximal and distal EHD) to correlate glucose metabolism with the expression of GLUT1 and HK2.
Results: SUV, T/L-ratio, and GLUT1 and HK2 expression did not differ as a function of histological grade and tumor location. GLUT1 and HK2 were expressed in 20 (76.9%) and 22 (84.6%) of 26 tumor biopsies, respectively. The GLUT1 score, SUV, and T/L-ratio increased, and the GLUT1 score, but not the HK2 score, correlated significantly with SUV (rho = 0.648) and T/L-ratio (rho = 0.703). There was no direct correlation between the expression of GLUT1 and that of HK2 (rho = 0.2046, P = 0.3161).
Conclusions: Although GLUT1 and HK2 regulate intracellular accumulation of FDG in many cancers, only GLUT1 expression was correlated with FDG uptake by EHD cancers.

• 出版日期2015-3