Apoptosis inducing and differentiation enhancement effect of oridonin on the all-trans-retinoic acid-sensitive and -resistant acute promyelocytic leukemia cells

作者：Gao, F.; Tang, Q.; Yang, P.; Fang, Y.; Li, W.; Wu, Y.^*

来源：International Journal of Laboratory Hematology, 2010, 32(1): E114-E122.

DOI：10.1111/j.1751-553X.2009.01147.x

• Oridonin
• apoptosis
• ROS
• leukemia
• differentiation

We investigated the effects of oridonin (Ori), a diterpenoid isolated from Rabdosia rubescens, on apoptosis and differentiation of all-trans-retinoic acid (ATRA)-sensitive (NB4) and ATRA-resistant (NB4-R1) cells. The results showed that reactive oxygen species initiates Ori-induced apoptosis. In addition, we found that neither Ori nor ATRA (10 nm) alone induced marked cell differentiation, while co-treatment of these two compounds can induce differentiation of NB4 and NB4-R1 cells which was accompanied by increased RAR alpha, C/EBP epsilon or C/EBP beta. This is the first report to show that RAR alpha could be accumulated by Ori which may be useful as a probe to investigate the mechanism of RAR alpha catabolism. These results suggest that Ori is a potential candidate for acute promyelocytic leukemia cancer therapy.

• 出版日期2010-2
• 单位上海市农业科学院; 温州医科大学; 上海交通大学