Analysis of multitemperature isothermal titration calorimetry data at very low c: Global beats van%26apos;t Hoff

作者：Tellinghuisen Joel

来源：Analytical Biochemistry, 2016, 513: 43-46.

DOI：10.1016/j.ab.2016.08.024

• ITC
• Data analysis
• Nonlinear least squares
• Global analysis
• Statistical errors

Isothermal titration calorimetry data for very low c (equivalent to K[M](0)) must normally be analyzed with the stoichiometry parameter n fixed - at its known value or at any reasonable value if the system is not well characterized. In the latter case, Delta H degrees (and hence n) can be estimated from the T-dependence of the binding constant K, using the van't Hoff (vH) relation. An alternative is global or simultaneous fitting of data at multiple temperatures. In this Note, global analysis of low-c data at two temperatures is shown to estimate Delta H degrees and n with double the precision of the vH method.

• 出版日期2016-11-15