Downregulation of CCR5 on activated CD4 T cells in HIV-infected Indians

作者：Dubey Shweta; Khalid Mohammad; Wesley Clement; Khan Samir Ali; Wanchu Ajay; Jameel Shahid^*

来源：Journal of Clinical Virology, 2008, 43(1): 25-31.

DOI：10.1016/j.jcv.2008.03.026

• HIV
• CCR5
• CXCR4
• co-receptor downregulation
• India

Background: HIV infection in India is unique as it occurs predominantly by CCR5-utilizing isolates that exhibit no co-receptor switch.
Objectives: To study HIV-1 co-receptor dynamics on T cells and monocytes following viral infection. Study design: HIV co-receptor expression was evaluated by flow cytometry on various cell subsets in HIV-infected Indians and in vitro in human peripheral blood mononuclear cells infected with CCR5- or CXCR4-utilizing HIV-1. Transfection of the T cell line CEM-CCR5 (which expresses CD4, CCR5 and CXCR4) with HIV-1 Nef or Vpu expression vectors, or treatment with recombinant soluble gp120 from CCR5- and CXCR4-tropic HIV-1, was carried out to determine their effects on co-receptor expression.
Results: Indian HIV patients had fewer CD4(+)CCR5(+) T cells and CCR5-expressing activated CD4(+) T cells, but higher CXCR4-expres sing activated CD4(+) T cells compared with controls. Expression of CCR5 was not different on monocytes in HIV patients as compared to controls. The CCR5 downregulation on T cells was HIV infection specific and was governed by the co-receptor-utilization phenotype of the virus. The Nef and soluble gp120 proteins induced CCR5 downregulation, the latter in a co-receptor-utilization phenotype specific manner.
Conclusions: The HIV-1 co-receptor dynamics in Indian patients is distinct from western patients and depends upon the virus surface protein. We propose this to be a viral survival strategy.

• 出版日期2008-9
• 单位河北医科大学