Activation of the blue opsin gene in cone photoreceptor development by retinoid-related orphan receptor beta

作者：Srinivas Maya; Ng Lily; Liu Hong; Jia Li; Forrest Douglas^*

来源：Molecular Endocrinology, 2006, 20(8): 1728-1741.

DOI：10.1210/me.2005-0505

Color vision requires the expression of opsin photopigments with different wavelength sensitivities in retinal cone photoreceptors. The basic color visual system of mammals is dichromatic, involving differential expression in the cone population of two opsins with sensitivity to short ( S, blue) or medium ( M, green) wavelengths. However, little is known of the factors that directly activate these opsin genes and thereby contribute to the S or M opsin identity of the cone. We report that the orphan nuclear receptor ROR beta (retinoid-related orphan receptor beta) activates the S opsin gene (Opn1sw) through binding sites upstream of the gene. ROR beta lacks a known physiological ligand and activates the Opn1sw promoter modestly alone but strongly in synergy with the retinal conerod homeobox factor (CRX), suggesting a cooperative means of enhancing ROR beta activity. Comparison of wild-type and mutant lacZ reporter transgenes showed that the ROR beta-binding sites in Opn1sw are required for expression in mouse retina. ROR beta-deficient mice fail to induce S opsin appropriately during postnatal cone development. Photoreceptors in these mice also lack outer segments, indicating additional functions for ROR beta in photoreceptor morphological maturation. The results identify Opn1sw as a target gene for ROR beta and suggest a key role for ROR beta in regulating opsin expression in the color visual system.

• 出版日期2006-8
• 单位NIH