A Graphene Oxide-Based Fluorescent Aptasensor for the Turn-on Detection of CCRF-CEM

作者：Tan, Jie; Lai, Zongqiang; Zhong, Liping; Zhang, Zhenghua; Zheng, Rong; Su, Jing; Huang, Yong; Huang, Panpan; Song, Hui; Yang, Nuo; Zhou, Sufang^*; Zhao, Yongxiang^*

来源：Nanoscale Research Letters, 2018, 13(1): 66.

DOI：10.1186/s11671-017-2403-3

• Aptamer
• Graphene oxide
• Leukemia
• CCRF-CEM

A convenient, low-cost, and highly sensitive fluorescent aptasensor for detection of leukemia has been developed based on graphene oxide-aptamer complex (GO-apt). Graphene oxide (GO) can absorb carboxyfluorescein-labeled Sgc8 aptamer (FAM-apt) by pi-pi stacking and quench the fluorescence through fluorescence resonance energy transfer (FRET). In the absence of Sgc8 target cell CCRF-CEM, the fluorescence is almost all quenched. Conversely, when the CCRF-CEM cells are added, the quenched fluorescence can be recovered rapidly and significantly. Therefore, based on the change of fluorescence signals, we can detect the number of CCRF-CEM cells in a wide range from 1 x 10(2) to 1 x 10(7) cells/mL with a limit of detection (LOD) of 10 cells/mL. Therefore, this strategy of graphene oxide-based fluorescent aptasensor may be promising for the detection of cancer.

• 出版日期2018-4-1
• 单位广西医科大学