An enzymatic method for determination of azide and cyanide in aqueous phase

作者：Wan, Nan-Wei; Liu, Zhi-Qiang; Xue, Feng; Zheng, Yu-Guo^*

来源：Journal of Biotechnology, 2015, 214: 27-32.

DOI：10.1016/j.jbiotec.2015.09.001

• Halohydrin dehalogenase
• Azide
• Cyanide
• Gas chromatograph

A halohydrin dehalogenase (HHDH-PL) from Parvibaculum lavamentivorans DS-1 was characterized and applied to determine azide and cyanide in the water. In this methodology, HHDH-PL catalysed azide and cyanide to react with butylene oxide and form corresponding beta-substituted alcohols 1-azidobutan-2-ol (ABO) and 3-hydroxypentanenitrile (HPN) that could be quantitatively detected by gas chromatograph. The detection calibration curves for azide (R-2= 0.997) and cyanide (R-2 = 0.995) were linear and the lower limits of detection for azide and cyanide were 0.1 and 0.3 mM, respectively. Several other nucleophiles were identified having no effect on the analysis of azide and cyanide, excepting nitrite which influenced the detection of cyanide. This was the first report of a biological method to determine the inorganic azide and cyanide by converting them to the measurable organics.

• 出版日期2015-11-20
• 单位浙江工业大学