Mass spectrometry-based proteomics: existing capabilities and future directions

作者：Angel Thomas E; Aryal Uma K; Hengel Shawna M; Baker Erin S; Kelly Ryan T; Robinson Errol W; Smith Richard D^*

来源：Chemical Society Reviews, 2012, 41(10): 3912-3928.

DOI：10.1039/c2cs15331a

Mass spectrometry (MS)-based proteomics is emerging as a broadly effective means for identification, characterization, and quantification of proteins that are integral components of the processes essential for life. Characterization of proteins at the proteome and sub-proteome (e. g., the phosphoproteome, proteoglycome, or degradome/peptidome) levels provides a foundation for understanding fundamental aspects of biology. Emerging technologies such as ion mobility separations coupled with MS and microchip-based-proteome measurements combined with MS instrumentation and chromatographic separation techniques, such as nanoscale reversed phase liquid chromatography and capillary electrophoresis, show great promise for both broad undirected and targeted highly sensitive measurements. MS-based proteomics increasingly contribute to our understanding of the dynamics, interactions, and roles that proteins and peptides play, advancing our understanding of biology on a systems wide level for a wide range of applications including investigations of microbial communities, bioremediation, and human health.

• 出版日期2012