Anti-HER2 VHH Targeted Magnetoliposome for Intelligent Magnetic Resonance Imaging of Breast Cancer Cells

作者：Khaleghi Sepideh; Rahbarizadeh Fatemeh^*; Ahmadvand Davoud; Hosseini Hamid Reza Madaah

来源：Cellular and Molecular Bioengineering, 2017, 10(3): 263-272.

DOI：10.1007/s12195-017-0481-z

• Magnetoliposome
• Specific cell targeting
• VHH
• Molecular imaging
• Breast cancer

The combination of liposomes with magnetic nanoparticles, because of their strong effect on T2 relaxation can open new ways in the innovative cancer therapy and diagnosis. In order to design an intelligent contrast agent in MRI, we chose anti-HER2 nanobody the smallest fully functional antigen-binding fragments evolved from the variable domain, the VHH, of a camel heavy chain-only antibody. These targeted magnetoliposomes bind to the HER2 antigen which is highly expressed on breast and ovarian cancer cells so reducing the side effects as well as increasing image contrast and effectiveness. Cellular iron uptake analysis and in vitro MRI of HER2 positive cells incubated with targeted nanoparticles show specific cell targeting. In vitro MRI shows even at the lowest density (200 Cells/mu l), dark spots corresponding to labeled cells which were still detectable. These results suggest that this new type of nanoparticles could be effective antigen-targeted contrast agents for molecular imaging.

• 出版日期2017-6