Antioxidant Activity Potential of Virginia (Flue-Cured) Tobacco Flower Polysaccharide Fractions Obtained by Ultrasound-Assisted Extraction

作者：Xu Chun Ping^*; Xiao Yuan; Mao Duo Bin

来源：Bioscience Biotechnology and Biochemistry, 2013, 77(10): 2100-2104.

DOI：10.1271/bbb.130461

• extraction
• optimization
• polysaccharide
• antioxidant activity
• tobacco flower

Ultrasound-assisted extraction was employed to extract polysaccharide from Virginia (flue-cured) tobacco flowers. The orthogonal matrix method (L-9(3)(4)) was used to determine the optimal extraction conditions as to ultrasound power, extraction time, ratio of solvent to solid, and extraction temperature at 300 W, 4 min, 35 (mL/g), and 70 degrees C respectively. The crude extract was successively purified by chromatography, yielding two major polysaccharide fractions, termed Fr-I and Fr-II. Both fractions are heteropolysaccharides, mainly containing glucose, mannose, and allose with an a-configuration. Thermo gravimetric analysis (TGA) indicated that the degradation temperatures (Td) of Fr-I and Fr-II were 185 degrees C and 190 degrees C respectively. The preliminary antioxidant activity test in vitro showed both fractions could potentialize the scavenging effect on hydroxyl and DPPH radicals in a dose-dependent manner. In conclusion, the two polysaccharides may be useful as naturally potential antioxidant agents for application in food and medicinal fields.

• 出版日期2013-10
• 单位郑州轻工业大学