Adiponectin Upregulates ABCG1 Expression Through Liver X Receptor alpha Signaling Pathway in RAW 264.7 Macrophages

作者：Liang Bin; Bai Rui; Han Yao-Xia; Guo Xiao-Hong; Xiao Chuan-Shi; Bian Yun-Fei^*

来源：Progress in Biochemistry and Biophysics, 2015, 42(9): 850-857.

DOI：10.16476/j.pibb.2015.0119

• adiponectin
• atherosclerosis
• ATP-binding cassette transporter G1
• reverse cholesterol transport

It has been well known that foam cells formation is one of the hallmarks of early atherosclerosis. Reverse cholesterol transport (RCT) pathway can inhibit the foam cells formation. ATP-binding cassette transporter G1 (ABCG1) plays a crucial role in RCT and anti-atherosclerosis, which mediates the efflux of cholesterol to HDL. Liver X receptor alpha (LXR alpha) can stimulate cholesterol efflux through ABCG1. It has been well known that adiponectin has cardiovascular protection. In this study, we attempted to clarify the effect of adiponectin on expression of ABCG1, and explored the role of LXR alpha in the regulation of ABCG1 in RAW 264.7 macrophages. The expression of ABCG1 and LXR alpha were examined by Real-time quantitative PCR and Western blot analyses. Cellar cholesterol efflux from THP-1 macrophage was analyzed by liquid scintillation counting assays. Our results showed that adiponectin increased ABCG1 expression at both the mRNA and protein levels in a dose-dependent and time-dependent manner. Consequently, adiponectin promoted cholesterol efflux in RAW 264.7 macrophages. Moreover, adiponectin up-regulated the expression of LXR alpha in a dose-dependent and time-dependent manner in RAW 264.7 macrophages. LXR alpha small interfering RNA completely abolished the promotion effects of adiponectin. In summary, adiponectin up-regulates ABCG1 expression via the LXR alpha pathway in RAW 264.7 macrophages.

• 出版日期2015-9
• 单位山西医科大学第二医院; 山西医科大学