Depth-resolved multimodal imaging: Wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography

作者：Chen Mingzhou^*; Mas Josep; Forbes Lindsey H; Andrews Melissa R; Dholakia Kishan^*

来源：Journal of Biophotonics, 2018, 11(1): UNSP e201700129.

DOI：10.1002/jbio.201700129

• brain tissue
• optical coherence tomography
• Raman spectroscopy
• turbid media

A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth colocalization obtained from the OCT, we can penetrate 1.2-mm deep into strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (polystyrene) with a spatial offset. Our approach is capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter at depth within a 0.6-mm thick brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.

• 出版日期2018-1